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TBS-Puffer
ββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ
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Contents
β’ Eigenschaften
β’ Einzelnachweise
ββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ
Eigenschaften
TBS-Puffer wird zur Untersuchung der Bindung und zur Lagerung von Proteinen sowie zur Immundetektion verwendet, darunter im Western Blot, im ELISA, im ELISPOT, in der Immunfluoreszenz und in der Immunhistochemie. TBS-Puffer besteht meistens aus 10 β 50 mM TRIS als Puffersubstanz und 150 mM Natriumchlorid fΓΌr eine physiologische IonenstΓ€rke.cite-ref-books-2xi9glcln2oc-99-1-0[1]cite-ref-books-3dnmdjwco6yc-473-2-0[2] Der pH-Wert wird je nach Protokoll mit verdΓΌnnter SalzsΓ€ure auf 7,2 bis 7,6 eingestellt.cite-ref-books-2xi9glcln2oc-99-1-1[1]cite-ref-books-meqjqc92esgc-411-3-0[3] Der pH-Wert nimmt pro Kelvin um 0,03 ab. Alternativ zum TBS-Puffer werden unter anderem Good-Puffer verwendet. TBS-Puffer mit Tween 20 wird als TBS-T-Puffer bezeichnet.
Einzelnachweise
cite-note-books-2xi9glcln2oc-99-11. β Academic Press: Antibodies in Cell Biology. Academic Press, 1993, ISBN 978-0-080-85935-4, S. 99.
cite-note-books-3dnmdjwco6yc-473-22. β M. A. Hayat: Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas. Academic Press, 2005, ISBN 978-0-080-49520-0, S. 473.
cite-note-books-meqjqc92esgc-411-33. β John Pound: Immunochemical Protocols. Springer Science & Business Media, 1998, ISBN 978-1-592-59257-9, S. 411.